Once elucidated, what can we potentially learn from the structure in terms of biological significance?Are there examples of drugs, or treatments that have only been made possible because of a known crystal structure?Written by: Verna Frasca During the development of proteins for use as biopharmaceuticals, the primary structure (amino acid sequence) is important in defining protein activity.
I have spent months as a student working on trying to form a tricky protein crystal.
But I have never actual had explained to me why the structure will be useful.
A clone was isolated from a human liver c DNA library.
The open reading frame encodes a 252-amino acid protein.
Proteins have attributes which can be susceptible to structural modifications, which can be affected by the above processes.
It is important that processes are designed to control the key protein attributes which are most critical for protein structure and activity.Generally speaking, why do people spend all the money on synchrotrons, laboratories, robots and so on, for a crystal structure?Protein structures, which can be obtained from protein crystals or from concentrated solutions of pure protein via NMR, are arguably the primary source of knowledge that we have about how genes perform their function on the molecular level.In this direction, we have generated the structure of CHPV N protein by homology modeling using SWISS-MODEL workspace and Accelrys Discovery Studio client 2.55 and mapped the domains of N protein using Pi SQRD.The interactions of N protein fragments with other proteins were determined by ZDOCK rigid-body docking method and validated by yeast two-hybrid and ELISA.The study revealed a unique binding site, comprising of amino acids 1–30 at the N terminus of the nucleocapsid protein (N1) that is instrumental in its interactions with N, P, M, and G proteins.